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Most protocol failures happen in the first 72 hours after mixing, when researchers unknowingly expose reconstituted peptides to temperature fluctuations that irreversibly denature the protein structure

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Korean J Parasitol 47(4):401 Radovanovi TB, Proki MD, Gavri JP, Despotovi SG, Gavrilovi BR, Borkovi-Miti SS, Pavlovi SZ, Saii ZS (2015) Glutathione-dependent enzyme activities and concentrations of glutathione, vitamin E and sulfhydryl groups in barbel ( Barbus barbus ) and its intestinal parasite Pomphorhynchus laevis (Acanthocephala)