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The plate was immediately incubated at 37 C in a humidified atmosphere with 5% CO 2 for 4 h

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Peroxidation of membrane results in huge compromise of the structural and functional integrity of the cell characterized by altered membrane permeability,17 dysfunctionality of membrane receptors,18 decreased activity of membrane-bound enzymes19 and increased membrane rigidity which decreases membrane fluidity.20 The possibility of free radicals directly attacking membrane proteins, induce lipid-lipid, lipid-protein and protein-protein cross linking also exist.21 Peroxynitrate (ONOO), a powerful oxidant is formed when O2 reacts with nitric oxide radical (NO) in the presence of arginine and NADPH-Oxidase enzyme

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C., Moody, A., Jaeck, D