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RNA was isolated from brain and islets using RNeasy micro kit (Qiagen), cDNA was synthesized from equal amounts of RNA using SuperScript III First-Strand Synthesis kit (Invitrogen) and gene expression was quantified using PowerUp Sybr Green Reagents (Invitrogen) according to the manufacturers instructions

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Now, with these drugs, were looking at 15%, and possibly even more. Rachel Brazil is a science writer based in London, UK