Briefly, tissues were homogenized in 500 L of RNAiso plus, with total RNA separated by mixing with 100 L of chloroform, and isolated by isopropanol precipitation
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For each peptide a paired Welch t-test p -value threshold of 0.05 was used to evaluate the significance of abundance variation GPX3 ELISA Following plasma thawing, a large subset of samples collected in 2021 from the discordant sibling cohort ( n = 174) were vortexed briefly and diluted 1:100 in ELISA sample buffer (NOVUS Biologicals)
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