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Lonardo, A., Ballestri, S., Marchesini, G., Angulo, P

CTK solution (0.25% trypsin + 1 mg/mL collagenase IV + 1 mM CaCl 2 + 20% KSR in DPBS ()) was prepared with trypsin ((2.5%), no phenol red, Thermo Fisher Scientific Inc.), collagenase (type IV, powder, Thermo Fisher Scientific Inc.), calcium chloride dihydrate (Fujifilm Wako Pure Chemical Corporation), Knock Out TM Serum Replacement (KSR, Thermo Fisher Scientific Inc.)

The chemiluminescent substrate (ThermoFischer Scientific Inc.) was applied to the blot according to the manufacturers recommendation

The use of histologically defined specific biomarkers in drug development with special reference to the glutathione S-transferases

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