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These promoter methylation changes correlated with transcriptomic changes, indicating a functional relationship between the epigenetic alterations and gene transcription

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Cancer treatments significantly elevate OS through multiple mechanisms: chemotherapeutic agents, particularly alkylating agents and platinum compounds, generate excessive ROS directly damaging sperm DNA and membranes (87, 88), while radiation therapy induces both acute and chronic OS states in testicular tissue, leading to prolonged spermatogenic dysfunction (95)

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Sequencing was done on Illumina HiSeq 4000 instrument, 75 bp PE runs, 20 10 6 reads per sample