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To identify kinase(s) phosphorylating Thr-157, we searched for the serinethreonine kinases belonging to Kinase, in vitro and/or top 5 kinases listed in the Kinase Prediction tool (61) for phosphorylation of Mak and Ick Thr-157 using PhosphoSitePlus, and picked up Ccrk, Map3k5, Map3k15, Map2k2, Stk16, Mos, and Stk32b (Fig 5D)

This complex mechanism of action requires caution in the use of nonselective agonists when researching relevant diseases [261, 262]

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MTs provide valuable insights into the biological impact of heavy metals by regulating their interference with crucial processes such as enzyme activity, gene expression, and protein functions, leading to a more accurate biological risk assessment of environmental contamination (Ruttkay-Nedecky et al., 2013

The UV-Vis absorption spectrum for D-CuP exhibited a distinct absorption peak at 584 nm, consistent with that of monomer copper peptide (M-CuP) (Fig