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Light intensity was determined by the experimental setting

Fluorescence was assessed using a cytoFLEX cytometer (Beckman Coulter)

Profiling the chemical nature of anti-oxytotic/ferroptotic compounds with phenotypic screening

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Likewise, assays of GCL and GS activity in the cytosol and chloroplast fractions of Arabidopsis leaf tissue show specific activities as follows: GCL cytosol 2.2 0.3 nmol min 1 mg total protein 1 (18% total activity) and GCL chloroplast 10.0 0.6 nmol min 1 mg total protein 1 (82% total activity)