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[67], overproduction of the gain-of-function GDH mutant in insulinoma MIN6 cells increased GSIS at low glucose concentrations (2 mmol/l and 5 mmol/l) without affecting insulin release at high glucose concentrations (825 mmol/l) [73]

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Structural verification (TEM): Transmission electron microscopy should confirm the presence of intact, closed vesicle structures displaying the characteristic "dark-light-dark" trilamellar membrane profile