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In the cell experiments, metformin was used at a concentration of 1 mM, simvastatin was used at 2 M, and when combined, the concentrations were 0.5 mM for metformin and 1 M for simvastatin

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& Schipani, E

After feeding all branching pathways, the excess of glycolytic flux is converted to lactate to preserve a sufficient pool of NAD+ for glycolysis and also to avoid the tricarboxylic acid (TCA) cycle inhibition due to excess NADH

Specifically, using GLOW as the control arm and KLOW as the treatment arm isolates KPVs contribution to inflammatory resolution, cell survival, and barrier function without confounding the angiogenic and matrix components present in both formulations