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Dehydration and electrolyte disturbances also tend to become more severe at night

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Common dosing considerations A good starting point for many people is: 20 to 30 grams of protein per meal using a shake or smoothie to fill gaps when appetite is low Many women do better focusing on consistency across the day rather than trying to overload one meal with protein

295 milliosmoles/L) containing (in mM): 138 NaCl, 5.6 KCl, 2.6 CaCl 2 , 1.2 MgCl 2 , 10 HEPES (adjusted to pH 7.4 with NaOH), and supplemented with 11.1 mM glucose, 20 l ml 1 FBS, 1 l ml 1 Pluronic F-127, and 1 M fura-2 acetoxymethyl ester (fura 2-AM