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These measurements allow the calculation of the ratio of GSH/GSSG, or the ratio of reduced to oxidized glutathione, in order to determine the oxidative stress present in cultured mammalian cells in response to a compound
Cells were seeded at 40 10 3 /well density onto a 96-well ImageLock tissue culture plate to reach a maximum density of 100% and incubated overnight in standard growth conditions
The pink color from cyanocobalamin actually provides some photoprotection by absorbing certain wavelengths
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