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Since the PHH used for the three experiments were from the same 10-donor cell batch, it was reasonable to assume that the data are biologically and statistically comparable and can be combined in a single data pool for concentrationresponse analysis (U.S

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also invented a chemical lactylation probe named sodium (S)-2-hydroxypent-4-ynoate (YnLac) which metabolically integrated into lactylated proteins, allowing them to be directly tagged with fluorescent or affinity markers for fluorescence visualization or proteomic analysis

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Correlation analysis between targeted and semi-targeted metabolomics datasets Metabolites detected in both semi-targeted and targeted LCMS datasets were matched based on metabolite identity and analyzed across overlapping samples