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5-HTP is converted into serotonin by the enzyme hydroxytryptophan decarboxylase and with the help of vitamin B6

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After pre-incubation, they were transferred to a 24-well plate supplemented with a fresh medium and topically exposed to 30 l of negative control (PBS), positive control (5% SDS causes keratinocyte death), and MB from 0.2 M to 500 M for 60 min at room temperature