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First, alcohol dehydrogenase converts alcohol into acetaldehyde

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The procedure was repeated for total enzymatic rates of product formed using reaction mixtures containing, in addition, aliquots (5 l) of glutathione transferase solution (protein concentration 810 g/ml)

Excess PFA was quenched with 100 mM NH 4 Cl for 20 min, then washed three times with PBS

[DOI] [PubMed] [Google Scholar] 33.Montecinos V., Guzmn P., Barra V., Villagrn M., Muoz-Montesino C., Sotomayor K., Escobar E., Godoy A., Mardones L., Sotomayor P., Guzmn C., Vsquez O., Gallardo V., van Zundert B., Bono M.R., Oate S.A., Bustamante M., Crcamo J.G., Rivas C.I., Vera J.C