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The stress-reducing and mood-enhancing properties of DSIP make it beneficial for individuals suffering from anxiety and depression

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3.3 Evaluation of cytokines and chemokines in tears during clinical study As detailed in the materials and methods section of the manuscript, the concentration of different cytokines and chemokines in tears was analyzed using flow cytometry at four study visits following randomization: V1, V3 (1 month after the initial control or the experimental formulation treatment), V4 (after the 1-week wash-out), and V6 (1 month after the crossover treatment)

Mistake 5: not accounting for dead space Every syringe has a small amount of "dead space" in the hub where the needle connects to the barrel