When combined with caloric restriction, they support an additional 2-4 pounds of weight loss per month compared to diet alone
The monovalent Fc fusions were produced in Expi293 cells by transient transfection adding a 2:1 ratio of the knob:hole constructs per the manufacturer instructions
Hu et al
And it was done to basically demonstrate what the steady-state fasting levels of glucose, insulin, BHB, and acetoacetate would be
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