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2001;281(1):E155-E161

Scaled liver extracts were transferred to bead blaster tubes with zircon beads and homogenized using D2400 BeadBlaster homogenizer (Benchmark Scientific) in cold 80% methanol spiked with 500 nM metabolomics amino acid standard mix (Cambridge Isotopes Laboratory)

Average daily calorie intake

Then, mass spectra data were processed and calculated using AB SCIEX analyst software (version 1.6.3, AB SCIEX, MA, USA) with calculation curves made with standards

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