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Phase Separation To separate the hydrolyzed FAs from non-hydrolyzed triolein centrifuge at 4C for 20 min 3,000 rpm (Beckman, TJ6)

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The choice between semaglutide and tirzepatide depends on factors like efficacy preferences, side effect profiles , insurance coverage, and provider recommendation rather than BMI differences

6c,d) demonstrating that the peptide stability improvement, conferred by the disulphide bridge, is translatable across species and independent of the different protease environments

Alvarez, V.E., Perry, B.E., Darling, L.E., and Elmore, D.E., FASEB J