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CRISPR-KO has been designed to slice out the mutational region in the genome, hence resulting in the synthesis of indels in the host genome, and the technique is gaining attention for its clinical use in patients with sickle cell disorder [156]
Briefly, binary masks of Syt1 and Cntfr positive regions were obtained by applying a 1 sigma radius Gaussian blur filter and setting a user-defined threshold
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