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doi: 10.1152/physrev.1983.63.4.1420 17

Beads were initially equilibrated in 20 mM Tris-HCl pH 8.0, 300 mM NaCl binding buffer, followed by incubation with 50 M His-tagged Syn constructs (Syn WT or Syn 1103 ) for 45 min at 4 C with gentle rotation

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For in-vitro laboratory research only not for human or veterinary use, diagnosis, or treatment

A patient who tolerates a graduated dose increase and reaches 2.4 mg consistently will achieve better long-term outcomes than one who starts high, experiences severe side effects, and must stop treatment