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Taking an integrated genetic and physiological approach, we treated seedlings of B73 wildtype and aminocyclopropane-1-carboxylic acid synthase (ACS) 2/6 double mutant, a maize genotype which lacks an enzyme in the ethylene biosynthetic pathway and therefore produce less ethylene, with aminocyclopropane-1-carboxylic acid (ACC), a precursor of ethylene

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