Cell culture and cell viability assays The human renal tubular epithelial cells (HK-2) and the human small intestinal epithelial cell line (HIEC-6) were purchased from Procell and Zhejiang Meisen Co, respectively
Yes, glutathione and Vitamin C are commonly taken together daily as part of a wellness and skincare routine
APX belongs to class I peroxidase family of proteins which are characterized by the presence of heme prosthetic groups
Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C
If you are taking standard ascorbic acid (vitamin C) and a standard glutathione capsule, your body may only be absorbing a small fraction of the dose