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To maintain their growth cycle, they require a steady supply of nutrients and oxygen both of which depend on red blood cells

The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

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Red blood cells are produced in the bone marrow, a process that needs Vitamin B12 and folic acid (another of the B group of vitamins)