Do not use: Sterile water for injection (lacks preservative, risking bacterial growth) Normal saline without bacteriostatic agent Distilled water or tap water Other diluents or carriers Step-by-Step Reconstitution Before starting: Gather all supplies: lyophilised IGF-1 vial, bacteriostatic water, sterile syringes (appropriate size), sterile needles (23-25 gauge), alcohol pads, weighing scale (if determining exact peptide weight) Work in a clean environment (ISO Class 6-7 clean room is ideal, but a clean work surface with proper aseptic technique is acceptable for research-grade work) Wash hands thoroughly Ensure the IGF-1 LR3 vial has reached room temperature (do not attempt reconstitution with cold vials, as this affects dissolution) Reconstitution procedure: Prepare the vial: Wipe the rubber septum (the rubber stopper) of the IGF-1 vial with an alcohol pad

Vegetarians and Vegans: Since Vitamin B12 is primarily found in animal products, individuals who follow plant-based diets may be at risk of deficiency and should consider supplementation
Good Lab Practices: Use appropriate PPE, follow institutional protocols, and reference your labs chemical hygiene plan
Sian J, Dexter DT, Lees AJ, et al
Published online September 1, 2025