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After cleavage with ULP1 to remove the His-SUMO tag, the eluted proteins were further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 GL column with an elution buffer containing 20 mM HEPES (pH 8.0) and 400 mM NaCl

This treatment also led to a decrease in nitrogen balance and reduced nitrogen content in various organs

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