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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice
doi:10.1021/bi9929711
In this location, the transporter is exposed to the external environment, and then, to bacteria (Rotoli et al., 2020)
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