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The human GCLM gene was amplified from the MGC clone MHS6278-202809187 and cloned into the SalI-opened pAAVS1 57 vector fused to mCherry by Gibson assembly

Naturally, vitamin B12 can be found in food such as meat, fish and dairy where b12 attaches itself to protein, which is separated by stomach acid during digestion, allowing it to be absorbed into the bloodstream

Tanwar V, Sachdeva J, Golechha M, et al

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