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First, the single-center study design limits the extrapolation of the findings to diverse populations and may introduce selection bias

revealed that the B-repeat segment of a cell surface protein of Staphylococcus aureus SdrD is capable of binding of 14 Ca 2+ ions, and that, based on the circular dichroism and Trp fluorescence analysis, concluded that although the metal-free form is strongly disorganized, the Ca 2+ -bound form is well folded (Josefsson et al., 1998)