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The formaldehyde-containing DMEM was removed and cells were washed 3 times with 20ml of ice-cold PBS supplemented with 1x complete protease inhibitor cocktail

The purified samples were dried (Thermo Savant SPD111 SpeedVac), lyophilized (Labcono Freezone 6 Plus), and then stored at 20 C until further analysis

Ace 125 mg (Acetaminophen) is thought to act primarily in the CNS, increasing the pain threshold by inhibiting both isoforms of cyclooxygenase, COX-1, COX-2, and COX-3 enzymes involved in prostaglandin (PG) synthesis

The variation in LAB antioxidant activity indicates that they are straindependent, which opens up the possibility of selecting bacteria with specific implementation and healthpromoting potential, as discussed in the following chapters

doi: 10.1038/s41467-017-02444-w 53 BhutiaYDGanapathyV