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Calcium influx in response to glutamate puffs at one dendritic location was mediated almost exclusively by mGluRs with negligible contribution of NMDARs, AMPARs, and VGCCs while, at another location on the same cell, calcium responses were mediated by NMDARs, AMPARs, and VGCCs, and sequential blockade of these sources gradually reduced and eventually eliminated calcium responses (Topolnik et al., To summarize, the requirement for [Ca 2+ ] i rise is one common condition for induction of long-term plasticity in inhibitory neurons

A high-throughput assay has been developed to improve this method

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The drugs, approved to treat diabetes and obesity as Ozempic, Wegovy, Mounjaro and Zepbound, are used by millions of Americans