semaglutide recombinant synthesis Enzymatic Strategies Behind Manufacturing How Semaglutide is Synthesized -

This strategy is disadvantageous because hydrazine, which is a toxic and dangerous reagent, is required for the removal of the Dde protecting group

DCM (240 ml) was added to obtained compound at 25-30C and stirred the mixture for 50 min at the same temperature

The fermentation may be carried out in fed-batch to produce expressed semapeptide

The process as claimed in claims 1 and 6, wherein the conversion of a peptide having amino acid sequence His-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu- Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly to Semaglutide is carried out by global deprotection of the compound to cleave the peptide chain from the Resin and deprotection of the protected amino acids by using cocktail mixture/cleaving reagent and reacting the obtained compound with compound of formula-3 in a solvent optionally in presence of a base and/or a coupling agent

In an aspect of the present invention, the expressed semapeptide can be cleaved enzymatically in vivo or in vitro (using either pure or partially purified fusion specific protease) by cleavage enzyme which cleaves at the junction between ubiquitin fusion tag and semapeptide to generate the semapeptide