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Slides were washed and nuclei were stained using DAPI (Thermo Fisher Scientific 62248, 1:2,000) for 5 minutes at room temperature, washed, and mounted (Abcam, ab104135)

Storage requirements Lyophilized (powder) 20C up to 24 months Reconstituted 28C up to 21 days Literature Peer-reviewed research for laboratory background Journal of Physiology Paris 2003 PMID 12735993 Sikiric S et al

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Pharmacologically, signaling studies demonstrate that tirzepatide mimics the actions of native GIP at the GIP receptor but shows bias at the GLP-1 receptor to favor cAMP generation over -arrestin recruitment, coincident with a weaker ability to drive GLP-1 receptor internalization compared with GLP-1

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