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A Nature Scientific Reports 2016 study showed GHK-Cu had no cytotoxicity to human keratinocytes at concentrations up to 5,800 M over 72 hours and did NOT induce inflammatory biomarkers (IL-1, IL-8, HSPA1A, FOSL1) unlike other copper compounds, concluding GHK-Cu has a low potential of inducing skin irritation

Diagnosing MCAS Diagnosing mast cell activation syndrome involves running lab tests to check for elevated levels of tryptase in the blood and for elevated levels of N-methylhistamine, 11B -Prostaglandin F2 (11B-PGF2) and/or Leukotriene E4 (LTE4) in the urine

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10.1093/toxsci/kfq175 29 KumarV.SinhaA