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This study demonstrated that cultured hepatocytes treated either with a precursor of GSH, such as L-cysteine or N-acetylcysteine, or with its soluble ethyl ester form, boosts the levels of cellular GSH, which upregulates CYP27A1, CYP27B1, and VDR but downregulates CYP24A1

The concentration of GSH can be determined either as an endpoint read of the color developed at 405 nm or by measuring the rate of color development at 405 nm

This -glutamylated sulfide compound is further deglutamylated and S -oxygenated to yield S -alk(en)yl-L-cysteine sulfoxide ( Figure 1 )