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Approximately 150 g of digested peptide from each sample was used for purification, which is within the C18 spin columns maximum binding capacity of 100 g (#744101, Harvard Apparatus, Holliston, MA, USA) and then lyophilized before being analyzed on the Orbitrap Fusion Tribrid mass spectrometer coupled with an Ultimate 3000 nano-UPLC system (Thermo Fisher Scientific, Waltham, MA, USA)

The stumps were approximately connected and subsequent atrophy was markedly attenuated, finally, they presented as performing similarly to a normal non-injured muscle, with no post-surgery leg contracture [44]

A precursor ion mass tolerance of 0.5 D was used to carry out this database search

All members of the PDGF family display potent angiogenic activity in vivo, however, the PDGF-B/PDGFR axis is the most characterized

Replace nebulizer cups and tubing according to manufacturer guidelines