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After TMB substrate solution is added, only those wells that contain Mouse NT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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Baseline Measurements (Pre- and Post-Washout): Post-Tirzepatide Baseline: After the Tirzepatide phase and before starting Retatrutide, researchers establish a new baseline for critical metabolic parameters (e.g., body weight, food intake, glucose levels, insulin, relevant hormone levels)