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Several studies have demonstrated that metformins stimulation of autophagy can result in increased apoptosis in cancer cells ( Moreover, metformins interactions with autophagy in glioma can be further supported by evidence showing that the drug, in combination with other therapies, can alter the tumor microenvironment ( 2.3 Necrosis Research has increasingly focused on the role of necrosis, especially necroptosis, in metformins therapeutic efficacy against glioma cells ( Furthermore, the combination of metformin with 2-deoxyglucose has demonstrated remarkable efficacy in promoting necrotic cell death in glioma cells ( Research has started to unveil the role of necrosis, particularly necroptosis, in metformins therapeutic efficacy

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Most importantly, upon confirming the decreased expression of GLUT1 or GLUT6, we observed a reduction in glucose uptake by the tumor cells (Fig