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When purified protein was incubated with the gels (Figure 2), a significant decrease in GST-GFP was found in the solution after two hours compared to gel-free controls in the 1:1 (p = 0.008), 5:1 (p = 0.005), 20:1 (p = 0.02), 40:1 (p = 0.02), and 80:1 (p = 0.02) PEGDA to glutathione ratio gels (Figure 2A)
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The problem with inexpensive glutathione supplements is that GSH is inherently unstable: the thiol group of the molecule oxidises relatively quickly to the inactive GSSG form upon exposure to heat, light or oxygen
These products are cleaved into glutamine, cysteine, and glycine under the action of 5-oxoalaninase and Cys-Gly peptidase, respectively, and then added to new GSH synthesis [32, 37] (summarized in Fig