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Therefore, to bridge the gap between mechanistic studies and clinical application, stratified randomized controlled trials that employ rigorously characterized microbial strains, standardized dosing protocols, and prespecified, patient-centered primary endpoints must be prioritized in future research

N-acetylcysteine treatment N-acetylcysteine was prepared in 0.9% normal saline and injected intraperitoneally into mice at a dose of 150 mg/kg daily for twenty-eight days during the induction of the social isolation stress model 50,51,52

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