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Doxorubicin stock solution (10 mM, 100% DMSO) was serially diluted in unbuffered seahorse assay medium to prepare 6 concentrations: 400, 300, 200, 100, 80 and 40 M

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Weight changes Weight effects are the slowest and least predictable

* Correspondence: Prawej Ansari, [email protected], [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Do not use extra dose to make up for a missed dose