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For anterograde tracing of the DMVsmall intestine circuit, rAAV-Ef1-DIO-hChR2(H134R)-mCherry-WPRE-pA (AAV-DIO-ChR2-mCherry, AAV2/9, 1.63 10 13 vg/mL, 200 nL) was injected into the DMV of ChAT-Cre mice