For comprehensive ingredient details on the protein blend, see our original coverage in Muscle Preservation Simplified: The Vitamin Shoppe's GLP-1 Support Protein with myHMB
At the end of the reaction, wash the resin with 200mLx3DMF, add 200mL DBLKx2 for deprotection 5min+7min, wash the resin with 200mLx5DMF, and continue to couple Fmoc-Gly-OH, Boc-Glu(OtBu)-OH, Fmoc-Leu-OH, Fmoc in sequence according to the peptide sequence -Tyr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tB
As can be seen from the comparison between example 1 and comparative example 1, the quality, such as purity, of the compound 2 'obtained by the process of comparative example 1 is obviously reduced (purity is reduced by 17%) by replacing the raw materials, the purity of the obtained intermediate compound 2' is 75.83%, the by-products are obviously increased, such as the residual amount of the raw material Arg34GLP-1 (9-37) is 18.35% (which is one order of magnitude higher than the impurity in example 1), the content of the double-modified impurity is 1.24%, which is much higher than the value in example 1, and particularly as shown in FIG
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Claim 5 : The process according to claim 1 and 2, which further comprises increase in accumulation of resulting Arg 34 GLP-l (9-37) peptide by fermentation
