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At the time of assay, the growth medium was replaced with HBSS, supplemented with 10 mM HEPES and 0.1% (w/v) ovalbumin and incubated at room temperature for 30 min

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Analytical assessment includes confirmation of peptide identity, purity verification using chromatographic methods, and evaluation of material consistency across production batches

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The Role of Hydration in Digestive Health Proper hydration is crucial for the digestive process, nutrient absorption, and microbiome health