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Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI
The strong correlation of "eating/licking paint" and pica with urinary excretion of some metals (especially lead, tin, thallium, antimony, and tungsten) suggests that pica may be a significant source of toxic metals, accounting for up to half of the children's exposure to them
Diagnosis Coeliac disease has similar symptoms to other conditions like Irritable Bowel Syndrome , leading to possible misdiagnosis if it is not tested properly
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Safety Adverse effects In general, high-dose lipoic acid administration has been found to have few serious side effects
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