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For inhibition of thioredoxin reductase or glutathione reductase activity, cells were subsequently treated with 1 M auranofin (Sigma Aldrich, Darmstadt, Germany) or 50 M 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) (Sigma Aldrich, Darmstadt, Germany) respectively for 1 h

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With regard to Wnt/-catenin signaling, the electrophilic ,-unsaturated ketone moiety of 4-HNE reacts with the side chain of -catenin to form covalent adducts that alter the turnover of -catenin

Once bound, RORt, along with recruited factors such as H3K27ac, drives strong expression of Th17 genes