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Given that GABA is generally sleep-promoting (Gottesmann, 2002), this suggests a potential behavioral homeostatic feedback loop, with nitrogen stress accumulation during chronic sleep loss promoting the synthesis of Put-GABA, and thus sleep

Parallel cultures diluted 1:6 in PBS were incubated at 37 C with shaking for 3 h, then treated with 1% glucose, 5 mM sodium pyruvate and 0.5% casamino acids, or vehicle for 30 min

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