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2.5 Reverse transcription and real-time quantitative PCR Total RNA was extracted by using RNAiso Plus reagent (Takara, 9109) following the protocol recommended by the manufacturer

Vitamin B12 should be supplemented in vegetarians who do not get adequate amounts of B12 from diet or patients consuming a low calorie/low protein diet

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Clinical trials such as REWIND have demonstrated that GLP-1 receptor agonists provide cardiovascular benefits while on treatment, and these advantages are not expected to persist when treatment is discontinued unless replaced with alternative therapy

Key point: These treatments are not mutually exclusive