At the molecular level, 5-amino-1MQ functions as a competitive inhibitor of NNMT, demonstrating remarkable potency with an IC of 1.2 0.1 M under standard assay conditions (50 M SAM, 100 M nicotinic acid). This represents a dramatic 10-fold improvement over the parent compound 1-methylquinolinium, achieved through strategic amino group substitution that enhances binding affinity to the NNMT active site. The compound's mechanism centers on preventing the methylation of nicotinamide to 1-methylnicotinamide (1-MNA), thereby preserving nicotinamide for recycling back to NAD+ through the salvage pathway. This intervention effectively blocks what researchers have termed the "NNMT metabolic drain" a process that simultaneously depletes NAD+ precursors and consumes cellular methylation capacity
Retinoids present a similar conflict
For example, B12 shots of 25-50 mg doses (nearly a whole monthly vial of ours) have been used to treat cyanide poisoning, neuropathy, and ALS
It also means the drowsiness effect will hit you during your waking hours, which most patients find disruptive
If your blood levels are lower than 200pg/mL or if you are diagnosed with pernicious anemia, your doctor may recommend B12 supplementation via injection
