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Before the ultrasound procedure, the mice were fasted for 8 h and intraperitoneally injected with 1.5% pentobarbital (45 mg/kg) 10 min prior to the procedure to anesthetize the animals

These features were defined by Ludwig et al

Alternative markers such as cystatin C-based eGFR or enzymatic creatinine assays may provide more accurate assessment when clinical concern exists, as recommended in KDIGO guidelines

After your consultation, once your clinician approves your plan, many patients receive their medication as early as the next day, shipped discreetly to your home